One-step purification of murine IgM and human α2-macroglobulin by affinity chromatography on immobilized snowdrop bulb lectin
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چکیده
منابع مشابه
One-step purification of murine IgM and human alpha 2-macroglobulin by affinity chromatography on immobilized snowdrop bulb lectin.
A new mannose-specific plant lectin (GNA) isolated from the snowdrop bulb was immobilized on Sepharose 4B and employed for the purification of certain glycoproteins with high-mannose type glycan chains. Murine IgM bound tightly to this column and was eluted with 0.1 M methyl alpha-D-mannoside whereas bovine and murine IgG were not bound. When a murine hybridoma serum containing IgM monoclonal a...
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ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 1988
ISSN: 0003-9861
DOI: 10.1016/0003-9861(88)90076-8